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How to aspirate a lymph node | VETgirl Veterinary Continuing Education Blog

In this VETgirl blog, we demonstrate the simple diagnostic technique of lymph nodes aspiration. This is an important diagnostic tool that can easily performed in general practice with just a 22 gauge needle directed into the enlarged lymph node. (Tip: try to avoid the direct center of the lymph node where there may be necrosis present). Some clinicians prefer to use a closed technique (with a 3-5 ml syringe attached). In this case, a dog presented with severe generalized lymphadenopathy; a preliminary diagnosis of lymphosarcoma was made based on clinical signs, physical examination findings, and in-house cytology. When aspirating, make sure to make a thin smear. Ideally, one should be stained in-house with Diff-Quick to assess for adequate sampling. When submitting slides, make sure that the slides are not submitted/shipper with a container of formalin, which can destroy the slides! Check out our VETgirl podcast on cytology sampling here for more great tips.

One thought on “How to aspirate a lymph node | VETgirl Veterinary Continuing Education Blog

  1. Hello, I came across your page searching for why my dogs lymph nodes are extremely swollen after aspiration. Do you have any information on this that you could share? Thank you

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